Electrostatic surface potential of Ca²⁺–CaM and TRPV1-CT peptide. (A) Surface of Ca²⁺–CaM (top) viewed from N- (left) and CT (right) face of equivalently positioned TRPV1-CT peptide (bottom). The molecular surface is colored according to the electrostatic potential from red (−6 kT/e) to blue (6 kT/e). Electrostatic potential surfaces were generated in PyMOL using APBS (Baker et al., 2001). (B) Cartoon representation of TRPV1-CT peptide against surface representation of binding pocket, showing the hydrophobic anchors: W787 (left) and L796 (right) positioned at the TRPV1-CT helix N and C termini, respectively.