Recovery of Rho-EGFP within discrete disc lobules after multiphoton photobleaching is monotonic and rapid. (A) Averaged end-on fluorescence images (top panels) are shown, and the traced disc periphery and incisure patterns with mFRAPb site are indicated by a red dot (bottom panels). (B) mFRAPb recovery curves. The recovery curves were fitted with a cylinder diffusion model (red line) described previously (Calvert et al., 2010). Green line is the data model difference. (C) D_lat plotted as a function of expression level and lobule area. The Pearson correlation coefficients (R) indicated poor correlation for both comparisons.