Figure 4. End-on imaging of... | Rockefeller University Press

Figure 4.

$Figure 4. End-on imaging of GFP-expressing rod outer segments allows incisure patterns to be visualized. (A) Images of retinal slices arranged with outer segments perpendicular to the chamber floor. (B) Averages of 10–20 488-nm confocal scans at single z planes 10–20 µm from the outer segment tip showing the incisure patterns in EGFP and Rho-EGFP– or Rho-PAGFP–expressing rods. (C) EM images showing the incisure patterns in frog rods (dark lines in the enface transmission electron micrograph and parallel lines indicated by the yellow arrows in the freeze fracture image). The size of the psf at the first sigma of its Gaussian intensity profile is shown approximately to scale (red ellipse). Enface EM reprinted with permission from Experimental Eye Research (Tsukamoto, 1987). Bar, ∼5 µm. Freeze fracture image reprinted with permission from Journal of Comparative Neurology (Corless et al., 1987).$

End-on imaging of GFP-expressing rod outer segments allows incisure patterns to be visualized. (A) Images of retinal slices arranged with outer segments perpendicular to the chamber floor. (B) Averages of 10–20 488-nm confocal scans at single z planes 10–20 µm from the outer segment tip showing the incisure patterns in EGFP and Rho-EGFP– or Rho-PAGFP–expressing rods. (C) EM images showing the incisure patterns in frog rods (dark lines in the enface transmission electron micrograph and parallel lines indicated by the yellow arrows in the freeze fracture image). The size of the psf at the first sigma of its Gaussian intensity profile is shown approximately to scale (red ellipse). Enface EM reprinted with permission from Experimental Eye Research (Tsukamoto, 1987). Bar, ∼5 µm. Freeze fracture image reprinted with permission from Journal of Comparative Neurology (Corless et al., 1987).

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