Figure 2. Light-scattering analysis of... | Rockefeller University Press

Figure 2.

$Figure 2. Light-scattering analysis of GluA2 and GluA3 ATD preparations. (A) SEC-MALS analysis for GluA2 ATD constructs differing in length and extent of glycosylation. UV absorbance at 280 nm is shown as a black trace; red circles indicate mol wt calculated from light-scattering and refractive index signals. (B) Size distributions by dynamic light scattering for the three GluA2S constructs with the mol wt and radius of each sample; no evidence of aggregation or higher ordered assemblies is apparent. (C) SEC-MALS analysis for the EndoH-digested GluA3 ATD expressed in GnTI− cells.$

Light-scattering analysis of GluA2 and GluA3 ATD preparations. (A) SEC-MALS analysis for GluA2 ATD constructs differing in length and extent of glycosylation. UV absorbance at 280 nm is shown as a black trace; red circles indicate mol wt calculated from light-scattering and refractive index signals. (B) Size distributions by dynamic light scattering for the three GluA2S constructs with the mol wt and radius of each sample; no evidence of aggregation or higher ordered assemblies is apparent. (C) SEC-MALS analysis for the EndoH-digested GluA3 ATD expressed in GnTI⁻ cells.