Figure 8.
![Figure 8. Voltage dependence of spermidine block in the D172N mutant. (A) Single-channel currents recorded in the presence of 0.1 µM [spermidine]i at +40, +50, and +70 mV. (B) Histograms for the substate and blocked times. (C) Voltage dependence of dissociation constants for the wild-type Kir2.1 (closed symbols) and the D172N mutant (open symbols).](https://cdn.rupress.org/rup/content_public/journal/jgp/139/3/10.1085_jgp.201110736/4/jgp_201110736r_fig8.jpeg?Expires=1767772338&Signature=2UezgsiHX~LP4qlteY5aSB6nraR-1iYiJKxXyn0uuPnMpptXxOVgEDhnbON-qpfwSBMT2TgERI7uY37FfYUGgxREwlTLw7ehy9qzRrJ33X8Em0blw~3PB4-cGNvIjS2Zu6vxryq3U2TeurBW9TK3I6vuLwR~4GndRoqrhHhOsLoMo6IscdFbx7f8m9HOAaoLtzbnv~pOQ6LJf~AxgxNERelgGlJVO4OsUX9FsnM-hCIux5FUZJKqP2a97ck7gxp3X9fFSBZBk~f-xx7PCafLPVWTxpeTQV2em38bZ4r43z65fuYE78lZOh-WEbTK4QAHwsMVyZBtmi8uPeD2r9BMvw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Voltage dependence of spermidine block in the D172N mutant. (A) Single-channel currents recorded in the presence of 0.1 µM [spermidine]i at +40, +50, and +70 mV. (B) Histograms for the substate and blocked times. (C) Voltage dependence of dissociation constants for the wild-type Kir2.1 (closed symbols) and the D172N mutant (open symbols).
