![Figure 9. Fixed charges in the transmembrane cavity increase affinity for K+ permeation. (A) i-V relationships for Kir1.1b N152D in inside-out patches with different symmetrical [K+]. (B) Conductance as a function of concentration for N152D. Lines represent the best fits to Eq. 1, with gmax = 39 ± 2 pS and Km = 4.7 ± 1.4 mM.](https://cdn.rupress.org/rup/content_public/journal/jgp/139/2/10.1085_jgp.201110727/4/jgp_201110727_fig9.jpeg?Expires=1767676484&Signature=wfa2SWDVZffjMiZFJ-qurDHqlMYdM39Gmr9skEwuA8wxLIo2KdPBvCjlBHd-oQoULvrhK3YGor7G~B6JmAb9Q~Vn4se0pJlGAYQKDt2H63WWBNfxf3txI3yvKx7WSCyPrRkJIV1g4bvFhux9RVkyXOZ1ZibLknHuefnOemc1mVoGIOH2uQej~X5hocJ3TS~PAMcq7fgbwWhUoQzXcnXeRuE-fSsF4TqxVQg3cGKfFpttWzTHxqQzCiy0cSoCrdYEFM0mU5lFsqh6Nz5f9Ev1eDuclFctT9UpshlDCeyyq9aX9yDvzLcv94Fjh5lG43DXwUnMNuKxkRLPZ1kcWyAXYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 9.
Fixed charges in the transmembrane cavity increase affinity for K+ permeation. (A) i-V relationships for Kir1.1b N152D in inside-out patches with different symmetrical [K+]. (B) Conductance as a function of concentration for N152D. Lines represent the best fits to Eq. 1, with gmax = 39 ± 2 pS and Km = 4.7 ± 1.4 mM.
