Inverse pitch (µm⁻¹) distributions for myoV-6IQ and myoV-4IQ. Histograms of the inverse pitch (µm⁻¹) calculated from individual actin filaments twirling along either myoV-6IQ (top) or myoV-4IQ (bottom) construct. The histograms are normalized. Actin filaments with an inverse pitch <±0.4 µm⁻¹ (pitch > 2.5 µm) are classified as non-twirlers. myoV-6IQ twirls with a left-handed pitch, with a mean value of 1.4 µm, whereas myoV-4IQ twirls either with a left- or right-handed pitch, with mean values of 1.2 and 1.0 µm, respectively.