Representative traces from the processive runs of rhodamine-labeled myoV-6IQ (left) and myoV-4IQ (right) motors along actin filaments. (A and B) Measured intensities: The total measured intensity is shown (A), along with its 12 component intensities (B). When the rhodamine probe bleached, all of the intensities dropped to baseline. (C–E) Calculation of the orientation of the probe in the actin frame of reference: Traces of the angles δ_P, microsecond wobble (C), β_P, probe axial angle (D), and α_P, probe azimuthal angle (E) in the actin coordinate frame.