Localization of IK in parotid clumps by immunofluorescence. Immunofluorescence experiments were performed as described in Materials and methods. (A and E) Transmitted laser light images of the parotid clusters. (B and F) The localization of IK is shown and illustrates a prominent PM distribution with enriched localization of channels to the apical domain of the cells. (C) The distribution of the tight junction marker ZO-1 in the clump of cells stained with IK antibodies in B. (G) The localization of InsP3R-3, known to localize to the ER juxtaposed to the apical PM, is shown in the same cells. (D) The overlap of IK and ZO-1 is shown. (H) The co-distribution of IK and InsP3R-3. Bar, 10 µm. Each image is a single z slice acquired at an approximately equatorial plane.
