GFP-ClC-1-FLAG fluorescence does not localize to triads after expression in adult skeletal muscle. (A) GFP fluorescence of a representative transverse section of TA muscle from an HSA^LR mouse 3 wk after injection of GFP-ClC-1-FLAG adenovirus. (B) RYR1 immunofluorescence of the same field as that shown in A. (C) Merged image of the images shown in A and B. (D and E) GFP fluorescence (D) and RYR1 immunofluorescence (E) images from representative longitudinal sections of TA muscle from HSA^LR mice 3 wk after injection of GFP-ClC-1-FLAG adenovirus.