Adenoviral-mediated expression of GFP-ClC-1-FLAG significantly reverses myotonia in TA muscle of HSA^LR mice. (A) GFP fluorescence of cross sections of HSA^LR TA muscle showing distribution of cytosolic GFP (left) and GFP-ClC-1-FLAG (right) 3 wk after injection. Note that the images represent regions of transduction at the site of injection and are not representative of the total muscle body. (B) Myotonia score (see Materials and methods) was significantly reduced in the TA muscle of HSA^LR mice after adenoviral-mediated expression of GFP-ClC-1-FLAG compared with contralateral TA muscle expressing cytoplasmic GFP. Non-transduced gastrocnemius muscle from HSA^LR mice served as an untreated positive control. Data are presented as mean ± SEM (6 and 14 experiments for WT and HSA^LR mice, respectively). A one-way ANOVA with Tukey’s post-hoc test was used for group comparisons. *, P ≤ 0.01.