Kinetic structure of TRPM8 channels at 20°C as indicated by 2-D dwell-time histograms and dependency difference plots. (A and B) 2-D dwell-time histograms plotting the square root of the number of interval pairs against the logarithm of the durations of adjacent open- and closed-interval pairs at 40 mV (A) and 100 mV (B). (C and D) Dependency difference plots at 40 mV (C) and 100 mV (D; see Materials and methods). Peaks represent an excess of open intervals adjacent to closed intervals for the indicated durations of adjacent intervals when compared with a theoretical 2-D distribution that was calculated assuming the open and closed intervals distribute randomly. The valleys represent a deficit of interval pairs with the designated open and closed durations compared with a random distribution.