Figure 8. Blocking of cysless/R352C... | Rockefeller University Press

Figure 8.

$Figure 8. Blocking of cysless/R352C channels by glibenclamide before and after MTS modification. (A and B) The blocking effect of 50 µM glibenclamide was enhanced after the channels were modified by MTSET and MTSEA. (C) Percentage change in mean current induced by MTSET (blue) and MTSEA (green). (D) The fraction of block, calculated as (1−Ig/I0) × 100% (Ig and I0 are the mean current in the presence of ATP and ATP plus glibenclamide, respectively), for cysless/WT channels (gray), cysless/R352C channels before modification (black) and after modification with MTSET (blue), and after modification with MTSEA (green). Star indicates a significant difference from cysless/WT channels.$

Blocking of cysless/R352C channels by glibenclamide before and after MTS modification. (A and B) The blocking effect of 50 µM glibenclamide was enhanced after the channels were modified by MTSET and MTSEA. (C) Percentage change in mean current induced by MTSET (blue) and MTSEA (green). (D) The fraction of block, calculated as (1−I_g/I₀) × 100% (I_g and I₀ are the mean current in the presence of ATP and ATP plus glibenclamide, respectively), for cysless/WT channels (gray), cysless/R352C channels before modification (black) and after modification with MTSET (blue), and after modification with MTSEA (green). Star indicates a significant difference from cysless/WT channels.