A single-channel recording showing that modification of 352C by MTSET and MTSEA affects both Po and single-channel amplitude. (A; top trace) A continuous recording showing the effects of MTSET or MTSEA on a single cysless/R352C channel. (Bottom traces) Expanded recordings for segments I–III marked in the top trace. A short part in segment I was further zoomed out in a. Corresponding all-points amplitude histograms of II and III were depicted. Blue and green lines are the results of Gaussian fitting. (B) Single-channel amplitude, Po, open time and closed time of MTSET- (blue) and MTSEA-modified (green) cysless/R352C channel, as determined by Gaussian fitting and kinetics analysis; n = 6.