Summary of the change in macroscopic mean currents after modification. The percentage change in the mean current is calculated as (I_∞/I₀−1) × 100%, where I₀ and I_∞ are the mean current before and after modification, respectively. The left column is the effect of MTSES (red), and the right is the effect of MTSET (blue). n = 3–8 for each construct. Stars indicate mutant channels exhibiting a reduced single-channel amplitude.