Figure 5.
Figure 5. E128K full-length KATP channels have decreased PIP2 response. (A) Traces of inside-out voltage clamp recordings from COSm6 cells transfected with Kir6.2 and WT (top) or E128K (bottom) SUR1. Patches were exposed to 1 mM (black line) or 3 mM (white bar) ATP or 5 µM PIP2 (striped bar). Bars: WT, 1 min and 1,000 pA; E128K, 1 min and 100 pA. Zero current is indicated by the dotted lines. (B) Average change of currents relative to the initial maximum current upon excision into ATP-free Kint/EDTA solution (arrows in A) after one or two sequential 30-s exposures to 5 µM PIP2. Error bars represent SEM; n = 8 (WT) and 12 (E128K) patches (*, P < 0.05; Student’s t test).

E128K full-length KATP channels have decreased PIP2 response. (A) Traces of inside-out voltage clamp recordings from COSm6 cells transfected with Kir6.2 and WT (top) or E128K (bottom) SUR1. Patches were exposed to 1 mM (black line) or 3 mM (white bar) ATP or 5 µM PIP2 (striped bar). Bars: WT, 1 min and 1,000 pA; E128K, 1 min and 100 pA. Zero current is indicated by the dotted lines. (B) Average change of currents relative to the initial maximum current upon excision into ATP-free Kint/EDTA solution (arrows in A) after one or two sequential 30-s exposures to 5 µM PIP2. Error bars represent SEM; n = 8 (WT) and 12 (E128K) patches (*, P < 0.05; Student’s t test).

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