Activation of MEND by diverse amphipaths in BHK cells. Cytoplasmic solutions contain 2 mM ATP and 0.2 mM GTP. (A) 5 µM U73122, but not the inactive “control” analogue, U73343, induces MEND within 30 s. (B) 20 µM edelfosine induces MEND. (C) 40 µM tamoxifen induces MEND. (D) 20 µM LPC induces MEND. Similar to detergent-induced MEND, LPC-induced MEND stops abruptly upon the removal of LPC.