TX100-induced MEND does not depend on canonical endocytic proteins. (A) TX100-induced MEND after extensive disruption of cytoskeleton, phosphoinositides, and ATP-dependent processes (5 µM latrunculin with 2 µM Ca, no ATP or GTP, and 2 mM AMP-PNP). FM 4–64 was applied three times before MEND. During the fourth application, 120 µM TX100 was applied, causing a 77% MEND response. Thereafter, 85% of FM dye fluorescence was retained, and FM dye was reapplied twice. The reversible FM fluorescence amounts to 15% of pre-MEND fluorescence. (B) Further characteristics of MEND induced by 120 µM. As indicated by the open bar graphs, TX100-induced MEND was not inhibited when the cytoplasmic solution contained 0.2 mM guanosine 5′-[γ-thio]triphosphate (GTPγS), 5 µM latrunculin with 2 mM AMP-PNP, 50 µM of an unmyristolated dynamin inhibitory peptide (DynPep; Tocris Bioscience), or 0.2 mM of the organic dynamin inhibitor, dynasore.