Effects of cMyBP-C reconstitution and PKA treatment in cTnI_Ala5/cMyBP-C^−/− skinned myocardium. Force–pCa (A) and k_tr–force (B) relationships in cTnI_Ala5/cMyBP-C^−/− preparations before (•; n = 7) and after reconstitution (○; n = 6) with cMyBP-C. Force–pCa (C) and k_tr–force (D) relationships in cMyBP-C–reconstituted cTnI_Ala5/cMyBP-C^−/− preparations before (•; n = 6) and after (○; n = 6) PKA treatment. All data are plotted as means ± SEM.