Figure 5.
Figure 5. Reconstitution of cTnIAla5/cMyBP-C−/− skinned myocardium with purified recombinant cMyBP-C. Representative silver-stained 10% SDS-PAGE gel of cTnIAla5 (lane 1) and cTnIAla5/cMyBP-C−/− preparations before cMyBP-C reconstitution (lane 2), after cMyBP-C reconstitution (lane 3), and after PKA treatment of reconstituted preparations (lane 4). Lane 5 shows the purified recombinant cMyBP-C protein expressed in baculovirus-infected Sf9 cells.

Reconstitution of cTnIAla5/cMyBP-C−/− skinned myocardium with purified recombinant cMyBP-C. Representative silver-stained 10% SDS-PAGE gel of cTnIAla5 (lane 1) and cTnIAla5/cMyBP-C−/− preparations before cMyBP-C reconstitution (lane 2), after cMyBP-C reconstitution (lane 3), and after PKA treatment of reconstituted preparations (lane 4). Lane 5 shows the purified recombinant cMyBP-C protein expressed in baculovirus-infected Sf9 cells.

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