Figure 2. Myofibrillar protein phosphorylation in untreated and PKA-treated WT, cTnIAla5, cMyBP-C−/−, and cTnIAla5/cMyBP-C−/− skinned myocardium. (A) Representative 12.5% SDS-PAGE gel of myofibrillar proteins isolated from untreated (−) and PKA-treated (+) skinned myocardium and stained with SYPRO-Ruby to detect total protein and Pro-Q Diamond to detect phosphorylated proteins. (B) Slopes of proteins (top panel; n = 8) and phosphoproteins (bottom panel; n = 8) determined from regression analysis of plots of area × average optical density versus protein loaded (µg). Each bar represents the mean, and the error bar represents the SEM. *, P < 0.05.
Figure 2.

Myofibrillar protein phosphorylation in untreated and PKA-treated WT, cTnIAla5, cMyBP-C−/−, and cTnIAla5/cMyBP-C−/− skinned myocardium. (A) Representative 12.5% SDS-PAGE gel of myofibrillar proteins isolated from untreated (−) and PKA-treated (+) skinned myocardium and stained with SYPRO-Ruby to detect total protein and Pro-Q Diamond to detect phosphorylated proteins. (B) Slopes of proteins (top panel; n = 8) and phosphoproteins (bottom panel; n = 8) determined from regression analysis of plots of area × average optical density versus protein loaded (µg). Each bar represents the mean, and the error bar represents the SEM. *, P < 0.05.

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