E357 differentiates coupled and uncoupled transport by Slc26a6. Current by wild-type or the indicated slc26a6 mutants was measured in oocytes clamped at 0 mV. NO₃⁻ and SCN⁻ currents were measured by replacing bath Cl⁻ with these anions. (A–C) Individual traces and the summary of the currents. Current and intracellular NO₃⁻ or SCN⁻ (D and E) were measured by Slc26a6 and the indicated mutants. For simplicity, the current traces are not shown for E, but they were similar to those in D. Results similar to those in D and E were obtained in four experiments, and the results in C are the mean ± SEM of the indicated number of experiments.