![Figure 1. The multiple transport modes of Slc26a6. All experiments are with oocytes expressing wild-type Slc26a6. (A) The simultaneous measurement of membrane potential (light gray trace), pHi (dark trace), and [Cl−]i (gray trace). (B) Measurement of the NO3− and SCN− currents in oocytes held at 0 mV, and typical I-Vs obtained with Cl−, NO3−, and SCN−. The I-Vs were obtained after a 5-min incubation with the respective anions to allow uptake of NO3− and SCN− to measure their inward current. (C and E) [Cl-]i and membrane potential measurements were used to monitor Cl−/formate− (C) and Cl−/oxalate= (E) exchange. (D) Cl− current in the presence and absence of 10 mM formate was measured at a holding membrane potential of −80 mV by altering bath Cl−. (F) The rates of Cl− transport in the presence of 1 mM oxalate= and 10 mM formate−. Results similar to those in A, B, and D were obtained in at least five experiments. Results in F are given as mean ± SEM of the number of experiments listed in the columns.](https://cdn.rupress.org/rup/content_public/journal/jgp/137/2/10.1085_jgp.201010531/4/jgp_201010531_gs_fig1.jpeg?Expires=1771659183&Signature=CphwjidT-A1TtnwaZnGIr0FNhMSYb100yw4~ubOd58XgdBdYf3bChgGVOi6f225V59pJskorWcXkeftb8BXmAGaAqrM-LokBVOFCy3UFkKod~aXpaBZ53E1yO69nhnJmHr5ZdeOsmE534uQLKJTPWjkVZZLoVsjKYqRGy9kKxGOcoX0mbHSordNLg4EwlBM9PP7yp-v3BbAQAYppr~fmsGXX3HQc66AvUWxXEeNTbNcGSC5cnNT8K347bAE4fp0KEjuQ-Ur7zBkgdalf-aMehk9o6MnoRioDpshS4pJXcfeIP4NWljXMc8n5YVqCIjAV~QKcgGfCegY0jnrT-~ySsQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The multiple transport modes of Slc26a6. All experiments are with oocytes expressing wild-type Slc26a6. (A) The simultaneous measurement of membrane potential (light gray trace), pHi (dark trace), and [Cl−]i (gray trace). (B) Measurement of the NO3− and SCN− currents in oocytes held at 0 mV, and typical I-Vs obtained with Cl−, NO3−, and SCN−. The I-Vs were obtained after a 5-min incubation with the respective anions to allow uptake of NO3− and SCN− to measure their inward current. (C and E) [Cl-]i and membrane potential measurements were used to monitor Cl−/formate− (C) and Cl−/oxalate= (E) exchange. (D) Cl− current in the presence and absence of 10 mM formate was measured at a holding membrane potential of −80 mV by altering bath Cl−. (F) The rates of Cl− transport in the presence of 1 mM oxalate= and 10 mM formate−. Results similar to those in A, B, and D were obtained in at least five experiments. Results in F are given as mean ± SEM of the number of experiments listed in the columns.
