Rescue of Slob in nerves rescues the alterations in synaptic transmission. (A) Sample EJC traces from WT, Slob-null, and two rescue lines (rescue in muscle or nerve, respectively). (B) Pooled data. The enhanced EJC peak amplitude in Slob-null flies (black bar) is rescued by adding Slob back to the presynaptic nerve (dark gray bar), but not to the postsynaptic muscle (light gray bar). (C) Sample mEJC traces from WT, Slob-null, and two rescue lines (rescue in muscle or nerve, respectively). (D) Pooled data. Enhanced frequency and amplitude of non-evoked synaptic transmission (black bars) are rescued by adding Slob back to the presynaptic nerve (dark gray bars), but not to the postsynaptic muscle (light gray bars).