Targeted nerve expression of Slob-RNAi enhances evoked and spontaneous synaptic transmission. (A) Sample EJC traces from one control line and two Slob-RNAi lines. The Slob-RNAi^nerve line expresses Slob-RNAi in nerves, whereas the Slob-RNAi^muscle line expresses Slob-RNAi in muscle. (B) Pooled data. Peak amplitude of EJC is increased significantly when Slob-RNAi is expressed in nerves (black bar) compared with the uncrossed WT¹ control (white bar). EJC in the Slob-RNAi^muscle line (gray bar) is not significantly different from the EJC in the WT¹ control. (C) Sample mEJC traces from one control line and two Slob-RNAi lines. (D) Pooled data. Frequency and amplitude of mEJCs are increased significantly in the Slob-RNAi^nerve line (black bar), but not in the Slob-RNAi^muscle line (gray bar), compared with the uncrossed WT¹ control (white bar). Refer to Materials and methods for the definition of the WT¹ line.