mEJC frequency and amplitude are increased in Slob-null flies. The muscle cell was voltage clamped at −60 mV as for Fig. 5, but no stimulus was delivered to the segmental nerve. (A) Sample mEJC traces from control and Slob-null flies in the absence or presence of 1 µM TTX. (B) mEJC frequency and amplitude distributions in WT^P41 and mut^IP1 flies. Bin sizes are 0.15 Hz and 0.05 nA, respectively. The distributions for mut^IP1 are shifted dramatically to higher frequency and amplitude. (C) Pooled data. Frequency and amplitude of mEJCs are increased significantly in mut^IP1 line (black bar) compared with WT^P41 flies (white bar) in the absence or presence of TTX. In mut^IP1rescue^all flies (gray bar), both mEJC frequency and amplitude are rescued to the level of the WT^P41 flies.