Evoked synaptic transmission is increased in Slob-null flies. (A) Representative recording of the muscle cell membrane potential during voltage clamping and sample traces. The EJC was measured with the muscle cell voltage clamped at −60 mV (top). The stimulation artifact arises from stimulation of the segmental nerve. Sample EJC traces from WT control, two Slob-null lines, and one ubiquitous rescue fly line are shown. (B) Pooled data. Peak amplitude of EJC is increased significantly in mut^IP1 and mut^K162 flies (black and dark gray bars) compared with the WT^P41 control (white bar). In addition, ubiquitous expression of Slob57 in a Slob-null background rescues synaptic transmission to the WT level (light gray bar).