Effects of DTT or H₂O₂ on L356C-E363C double-mutant channels. (A–C) Macroscopic currents at pH_o 8 (left) and corresponding G-V curves (right; mean ± SEM; n = 5) for L356C-E363C mutant channels after the application of DTT (A), H₂O₂ (B), or GO (C). (D) Percent change of I₋₂₀₀/I₂₀₀ values (mean ± SEM; n = 5) at pH_o 8 of the L356C-E363C double-mutant channels after exposure to the reagents indicated. Positive or negative changes signify increases or decreases in the I₋₂₀₀/I₂₀₀ value, respectively. *, P < 0.001 (one-way ANOVA) with respect to the control mean.