Figure 4.
![Figure 4. Ca2+ and pH effects on ClC-Ka at mixed conditions. (A and B) Effect of [Ca2+]ext on ClC-Ka measured at pHext 8 (A, n = 6) and pHext 6.5 (B, n = 4). Currents at 60 mV were normalized to values measured in 10 mM [Ca2+]ext and plotted versus [Ca2+]ext. (C) Effect of pHext on ClC-Ka in 0.5 mM Ca2+ (n = 3). Currents at 60 mV were normalized to values measured at pH 7.3 and plotted versus pHext. Lines represent the theoretical predictions obtained using Eqs. 1 and 2 as described in Results using the same parameters obtained from the fit in the standard conditions (i.e., pK = 7.11; KC = 19.6 mM, KO = 1.5 mM, and r0 = 434).](https://cdn.rupress.org/rup/content_public/journal/jgp/136/3/10.1085_jgp.201010455/4/jgp_201010455_lw_fig4.jpeg?Expires=1773567655&Signature=h7S3~Mnf7USQ~PmtBrYvHeCTwH60bKrqUaCxa~5FZ1Ji~RW-bHQoGKfiWXgw7EVJL3DkthrGzYON4FXOhK3nwQ8R4pBfQ-WMAMIhgatkWKsfO~MYIkewC7sFV8AUMmYVuE-VqJtuu8EsyKzxRb1pfG--IoJ7ru67gBI-9S1dmsX2M00xVGiOQsy~rsssrtof2oMBG7-HSmgGZAYgzCZuuuST~YEXGi1QSLKookj1l6Q2zDVwbK-gAeuq2KqnIPw5gWV1Qu3YDJl9w~bTQYbKZxk9Jz1VNauAzzdCG8scvbDKata2wXP1B-XRVw-JzXRiXl2okrVeqWVmmhIkRqWEiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ca2+ and pH effects on ClC-Ka at mixed conditions. (A and B) Effect of [Ca2+]ext on ClC-Ka measured at pHext 8 (A, n = 6) and pHext 6.5 (B, n = 4). Currents at 60 mV were normalized to values measured in 10 mM [Ca2+]ext and plotted versus [Ca2+]ext. (C) Effect of pHext on ClC-Ka in 0.5 mM Ca2+ (n = 3). Currents at 60 mV were normalized to values measured at pH 7.3 and plotted versus pHext. Lines represent the theoretical predictions obtained using Eqs. 1 and 2 as described in Results using the same parameters obtained from the fit in the standard conditions (i.e., pK = 7.11; KC = 19.6 mM, KO = 1.5 mM, and r0 = 434).
