Figure 2.
![Figure 2. pHext dependence of ClC-Ka and ClC-Kb. (A) Voltage clamp traces of ClC-Ka measured at different pH as indicated, in 10 mM [Ca2+]ext. (B) Effect of pHext on ClC-Ka (circles; n = 7). Normalized currents were calculated as for Fig. 1. I/I10 mM Ca2+, pH 7.3, is plotted versus pH. The line represents the fit obtained using Eq. 1 as described in Results. (C) Superimposed traces measured at pH 7.0 (black line) and pH 8.0 (gray line) measured from the same oocyte. Currents were scaled to the steady-state current at 60 mV. Horizontal bar, 200 ms. (D) Traces of ClC-Kb measured at different pH as indicated, in 10 mM [Ca2+]ext. (E) Effect of pHext on ClC-Kb (squares; n = 4). Horizontal bars, 200 ms. Vertical bars, 5 µA.](https://cdn.rupress.org/rup/content_public/journal/jgp/136/3/10.1085_jgp.201010455/4/jgp_201010455_gs_fig2.jpeg?Expires=1773496539&Signature=fkyb2eaVTWwz64vpRngEOFlRQdqBhAB3VXDpCAbYLceE6rWyOXBO9zQIYXw85DbLdxhIxwXG0Lj7~dnOpYyeAwzh7TNfe-kOoI-Pb1wrVWN1TulnmpiIzNSYRHb3rnr~69JcovxvM1sDS0tHcP3jF08tbndG9lihcS~dvMzdvqq3~y5Z4ZbmTvJOZmJ9vqI-fGFUoroBCsBBILdj~x9vOGd3YKI3qAPiEF4AT5totH7~~v8hu9I4UkOIfEb77rjyQeuT3olAS0CFYlBzIJ7Ap2bjfwdijzGnhnOcoD6r5jD1X67xSoJ4Y~NAPQH6pZyEsk7hOqBb3rEN1gDEq6XdLA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
pHext dependence of ClC-Ka and ClC-Kb. (A) Voltage clamp traces of ClC-Ka measured at different pH as indicated, in 10 mM [Ca2+]ext. (B) Effect of pHext on ClC-Ka (circles; n = 7). Normalized currents were calculated as for Fig. 1. I/I10 mM Ca2+, pH 7.3, is plotted versus pH. The line represents the fit obtained using Eq. 1 as described in Results. (C) Superimposed traces measured at pH 7.0 (black line) and pH 8.0 (gray line) measured from the same oocyte. Currents were scaled to the steady-state current at 60 mV. Horizontal bar, 200 ms. (D) Traces of ClC-Kb measured at different pH as indicated, in 10 mM [Ca2+]ext. (E) Effect of pHext on ClC-Kb (squares; n = 4). Horizontal bars, 200 ms. Vertical bars, 5 µA.
