Figure 3. Effect of lidocaine on gating currents of WT and mutant sodium channels. (A; left) Representative ON gating currents of the WT channels and their integrals before and after 10-mM lidocaine application. (Right) Steady-state Q-V relationships of the WT channel before (filled symbols) and after (open symbols) lidocaine application. The gray dashed line represents the average reduction in the total gating charge after lidocaine application. The data represent mean ± SEM of at least three independent measurements. The line represents the best fit of the averaged data to a single Boltzmann function. (B) Steady-state Q-V relationships of mutant channels before (filled symbols) and after (open symbols) 10-mM lidocaine application. The gray dashed line represents the average reduction in the total gating charge after the application of lidocaine to the WT channel. The data represent mean ± SEM of at least three independent measurements, and the smooth lines represent the best fits of the averaged data to a single Boltzmann function. (C) Percentage reduction in the total gating charge upon the application of lidocaine in the WT and mutant channels. The total charge movement was measured by a pulse to +40 mV from −130 mV. The data represent mean ± SEM of at least three independent measurements.
Figure 3.

Effect of lidocaine on gating currents of WT and mutant sodium channels. (A; left) Representative ON gating currents of the WT channels and their integrals before and after 10-mM lidocaine application. (Right) Steady-state Q-V relationships of the WT channel before (filled symbols) and after (open symbols) lidocaine application. The gray dashed line represents the average reduction in the total gating charge after lidocaine application. The data represent mean ± SEM of at least three independent measurements. The line represents the best fit of the averaged data to a single Boltzmann function. (B) Steady-state Q-V relationships of mutant channels before (filled symbols) and after (open symbols) 10-mM lidocaine application. The gray dashed line represents the average reduction in the total gating charge after the application of lidocaine to the WT channel. The data represent mean ± SEM of at least three independent measurements, and the smooth lines represent the best fits of the averaged data to a single Boltzmann function. (C) Percentage reduction in the total gating charge upon the application of lidocaine in the WT and mutant channels. The total charge movement was measured by a pulse to +40 mV from −130 mV. The data represent mean ± SEM of at least three independent measurements.

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