Figure 2.
KcsA E71A channels transfer from NABBs to lipid bilayers. (A) Cartoon representation of KcsA transfer from NABBs into lipid bilayers. The NABB model was produced using the University of California, San Francisco, Chimera package using HDL particle coordinates provided by J.P. Segrest [University of Alabama at Birmingham, Birmingham, AL] and KcsA coordinates deposited in the Protein Data Bank (accession no. 3EFF). The BLM was constructed using Visual Molecular Dynamics software. (B) Spontaneous transfer of channels as a function of time from NABBs after adding NABBs with or without KcsA E71A to BLMs. The same amount of KcsA, from a 2-µg/ml stock, was added in each case.