Figure 3.
Function of N terminus chimeras of CRACM1 and CRACM3. (A) Average CRAC current densities at −80 mV induced by 20 µM IP3 in stable STIM1-expressing HEK293 cells transiently overexpressing CRACM1-wt (black; n = 43), CRACM3-wt (red; n = 70), CRACM1-N(M3) (green; n = 5), and CRACM3-N(M1) (blue; n = 5). Error bars indicate SEM. (B) Average I-V relationships of CRAC currents extracted from representative HEK293 cells shown in A and obtained at 120 s. Data represent leak-subtracted current densities (pA/pF) evoked by 50-ms voltage ramps from −150 to +150 mV.
