ASL measurements in pig trachea. (A; left) SLRM images at indicated times after the addition of 20 µl PBS containing rhodamine B-dextran. Bar, 40 µm. (Middle left) Corresponding intensity versus distance profiles perpendicular to laser line. (Middle right) Corresponding confocal z-scans. Bar, 40 µm. (Right) Time course of ASL depth after the addition of 20 µl PBS containing rhodamine B-dextran. Mean ± SEM of three tracheal fragments. (Inset) Histogram of ASL depths from 50 measurements across SLRM image at indicated times. (B; left) SLRM images before and 30 min after the addition to the perfusate of 20 µM forskolin, 100 µM IBMX, and 1 mM carbachol. Bar, 40 µm. (Middle left) Corresponding intensity versus distance profiles perpendicular to laser line. (Middle right) Corresponding confocal z-scans. Bar, 40 µm. (Right) Time course of ASL depth after the addition of agonists. Mean ± SEM of three tracheal fragments. SLRM images were contrast-adjusted to obtain sharpest intensity versus distance profiles.