Figure 4.
Spontaneous calcium signals in MNTB astrocytes. (A) Overview of the MNTB region of a GFAP-mRFP1 transgenic mouse brainstem slice showing mRFP1 fluorescence. Bar, 200 µm. (B) On the left, a cell (marked by a box in A) is displayed in higher magnification. The top image is the overlay of mRFP1 expression and Fluo-4 staining, the bottom image is the Fluo-4 staining alone. Bar, 20 µm. On the right, the Fluo-4 fluorescence change (F/F0) from that cell is shown.
