Currents from E1C/R1E and E1C/R1E+KCNE1. (A, left) Same protocol used as in Fig. 1 B. (Right) E1C/R1E+KCNE1 currents after superfusion of MTSES⁻ or MTSET⁺. (B; left) Currents from E1C/R1E/Q3R and E1C/R1E/Q3R+KCNE1. Same protocol as in A. (Right) G-V relationships from E1C/R1E/Q3R with or without KCNE1 were plotted with G-V relationships of WT Kv7.1 (left gray line) or WT Kv7.1+KCNE1 (right gray line). Error bars represent standard error of the means. Scale: E1C/R1E, 0.7 µA; E1C/R1E+KCNE1, 12 µA; E1C/R1E/Q3R, 1 µA; E1C/R1E/Q3R+KCNE1, 5 µA. (C; left) E1C/R1E/Q3R+KCNE1 currents after superfusion of MTSES⁻ or MTSET⁺. Same protocol as in Fig. 5 A. Scale: top, 5 µA; bottom, 2 µA. (Middle) Peak current amplitudes plotted against time. Error bars represent standard error of the means. (Right; top) Normalized peak current amplitude after various MTS treatments. (Right; bottom) G-V relationships before and after MTS treatment. Red line indicates the G-V before MTS treatment. Error bars represent standard error of the means.