The MscL (V23T) channel quickly adapts in excised patches but shows no adaptation in whole cell mode. (A) Fast adaptation in response to a step of pressure in excised patch. (B) Responses to two ramps separated by a 45-s near-threshold conditioning pulse (ramp-step-ramp). The response to the second ramp is right-shifted by 30 mmHg. (C) Whole spheroplast response to a step shows no adaptation. (D) The ramp-step-ramp response of whole spheroplast indicates no midpoint shift.