MscS and MscL both adapt, displaying similar right shifts of activation curves with similar time courses. (A) A pressure ramp response of an excised patch containing both MscS and MscL, followed by a prolonged (59-s) step of subthreshold pressure with a repeated ramp in the end. The midpoints scored with the second ramp are noticeably higher. (B) Superimposed traces in response to a 500-ms ramp show that the midpoint shift for both channels increases gradually with the duration of the subthreshold step varied between 0.2 and 59 s. (C) Plot of activation midpoints as a function of intervening step duration. The midpoints for MscS (bottom) and MscL (top) change concomitantly with characteristic times of ∼2 s.