![Figure 3. Inhibition of CLC-5 currents in SCN−. (A) CLC-5 currents recorded from the same oocyte in various pHex. Scale bars represent 2 µA and 10 ms. (B) Current–voltage plots for the currents shown in A. Squares, pH 9; circles, pH 7.5; triangles, pH 6.0; diamonds, pH 4.5. Solid lines hold no theoretical meaning. (C) Plot of normalized currents at +60 (filled circles) and +110 (open circles) mV as a function of pH. Solid lines are fits to Eq. 3 as described in Results, with K = 6.0 × 10−7, Imax = 1.04 and Imin = 0.25 at +60 mV, and K = 2.1 × 10−6, Imax = 1.05 and Imin = 0.31 at +110 mV. Dotted lines represent the fits at +110 (gray) and +60 (black) mV at 100 mM [Cl−]ex. (D) Plot of the mean values of the pK obtained as in C, versus voltage. Solid line is a fit to pK(V) = pK(0) + zFV/RT, where pK(V) is the pK at voltage V, pK(0) = 6.9 is the pK extrapolated at V = 0, z = 0.63 is the fraction of the membrane potential acting on the blocking ion, and F, R, and T have their usual meanings.](https://cdn.rupress.org/rup/content_public/journal/jgp/135/6/10.1085_jgp.201010428/4/jgp_201010428_gs_fig3.jpeg?Expires=1767775720&Signature=2J2Umfx2YWr2S~Dw0tAGo5-XUVqt~SALList3O3vdrlzwhU-wavih0rYFFyCJ3voeA0ue~f0f9nVNU4-6TeP0R80u~pNHhqiLK2fAmbF4GDr12aes3esNDV8hcXsnY8wgL8bJ3~B6yte1EMh8zliv6H4jBHMUQnBjsd6x-vn0KWcxjV3m8NTa-fTKA6RcsjqtUr7Tq3uaGcE0pgd1DTaBy0UevApNLEa6oKLskHhvz8CgeWy53Xe5Q19nkzjk9IceDRE9bxnwPqGgBzj6eJxn6Kvetdk4uxUJyEcbP1GJxz3KHd5l1qUDpPdmOyZYV574SyRWXA1MbPKMtelLylLlQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of CLC-5 currents in SCN−. (A) CLC-5 currents recorded from the same oocyte in various pHex. Scale bars represent 2 µA and 10 ms. (B) Current–voltage plots for the currents shown in A. Squares, pH 9; circles, pH 7.5; triangles, pH 6.0; diamonds, pH 4.5. Solid lines hold no theoretical meaning. (C) Plot of normalized currents at +60 (filled circles) and +110 (open circles) mV as a function of pH. Solid lines are fits to Eq. 3 as described in Results, with K = 6.0 × 10−7, Imax = 1.04 and Imin = 0.25 at +60 mV, and K = 2.1 × 10−6, Imax = 1.05 and Imin = 0.31 at +110 mV. Dotted lines represent the fits at +110 (gray) and +60 (black) mV at 100 mM [Cl−]ex. (D) Plot of the mean values of the pK obtained as in C, versus voltage. Solid line is a fit to pK(V) = pK(0) + zFV/RT, where pK(V) is the pK at voltage V, pK(0) = 6.9 is the pK extrapolated at V = 0, z = 0.63 is the fraction of the membrane potential acting on the blocking ion, and F, R, and T have their usual meanings.
