Figure 2.
![Figure 2. Proton inhibition of CLC-5 currents in low extracellular Cl−. (A) Plot of normalized currents at +60 (filled circles) and +110 (open circles) mV as a function of pH. Solid lines are fits to Eq. 3 as described in Results, with K = 7.1 × 10−7, Imax = 0.98 and Imin = 0.25 at +60 mV, and K = 2.1 × 10−6, Imax = 0.97 and Imin = 0.24 at +110 mV. Dotted lines represent the fits at +110 (gray) and +60 (black) mV at 100 mM [Cl−]ex. (B) Plot of the mean values of the pK obtained as in A, versus voltage. Solid line is a fit to pK(V) = pK(0) + zFV/RT, where pK(V) is the pK at voltage V, pK(0) = 6.7 is the pK extrapolated at V = 0, z = 0.56 is the fraction of the membrane potential acting on the blocking ion, and F, R, and T have their usual meanings.](https://cdn.rupress.org/rup/content_public/journal/jgp/135/6/10.1085_jgp.201010428/4/jgp_201010428_gs_fig2.jpeg?Expires=1767775718&Signature=bs7ATjOH9lteNk5hPeNLFYqYuK3x1lB466yOy2Z-Ke58VXKfObN7h8vuzCLTxA20M~6kPLZZ~8oHJvkwQNfkfi~0QGRkE-o55KH2lHYRxX2lFCWP0CGhrGfFi03vzY993NhdH5ftMmdCy-2VbdbICRQ-R5xmnzSdWsJR8arIWoTGtQnhZN1~8bJWJnH6jFzWERZSUHHhg9QhLQwixysteMtuz9rmJKxxp2R84GZ54Xz6Wml8soLpcqCYUvwTcNfsFncA9dJdUUREq5g5RDU4U2Lut3eCP6I~nC~EFCtvuiTLLnWl8B1~9WDWoatvTlSL52dHpkPJo-aLLbIS6VtQog__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Proton inhibition of CLC-5 currents in low extracellular Cl−. (A) Plot of normalized currents at +60 (filled circles) and +110 (open circles) mV as a function of pH. Solid lines are fits to Eq. 3 as described in Results, with K = 7.1 × 10−7, Imax = 0.98 and Imin = 0.25 at +60 mV, and K = 2.1 × 10−6, Imax = 0.97 and Imin = 0.24 at +110 mV. Dotted lines represent the fits at +110 (gray) and +60 (black) mV at 100 mM [Cl−]ex. (B) Plot of the mean values of the pK obtained as in A, versus voltage. Solid line is a fit to pK(V) = pK(0) + zFV/RT, where pK(V) is the pK at voltage V, pK(0) = 6.7 is the pK extrapolated at V = 0, z = 0.56 is the fraction of the membrane potential acting on the blocking ion, and F, R, and T have their usual meanings.
