Figure 9.
Figure 9. MTS-reactive positions are located on one face of the H8-H9 hairpin in the aqueous crystal structure. The figure shows the hairpin consisting of residues 580–612, plus the flanking residues 579 and 613 (in a truncated rendering). The residues colored red or orange are those that, when mutated to cysteine, evoke a single-channel conductance change upon reaction with MTS derivatives. (Residue A582 is colored orange to distinguish it from residue T581.) Three additional reactive positions, T573, G577, and N578, are not shown because they are not part of the hairpin in the crystal structure. Residues 579–613 were excerpted from the crystal structure of whole colicin Ia (Wiener et al., 1997), available from the Protein Data Bank (Berman et al., 2000) as file 1CII, and displayed as a ribbon model using RasMol (v2.7.1).

MTS-reactive positions are located on one face of the H8-H9 hairpin in the aqueous crystal structure. The figure shows the hairpin consisting of residues 580–612, plus the flanking residues 579 and 613 (in a truncated rendering). The residues colored red or orange are those that, when mutated to cysteine, evoke a single-channel conductance change upon reaction with MTS derivatives. (Residue A582 is colored orange to distinguish it from residue T581.) Three additional reactive positions, T573, G577, and N578, are not shown because they are not part of the hairpin in the crystal structure. Residues 579–613 were excerpted from the crystal structure of whole colicin Ia (Wiener et al., 1997), available from the Protein Data Bank (Berman et al., 2000) as file 1CII, and displayed as a ribbon model using RasMol (v2.7.1).

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