Charge neutralization of R463 and R467 is predicted to weaken channel–PIP₂ interactions, but charge reversal is predicted to strengthen them. Shown for (A) R463Q, (B) R463E, (C) R467Q, and (D) R467E are an expanded view of the results of the docking simulation of GPMI-P₂ docked to the top of the corresponding helix A-B linker, the electrostatic profile of the putative PIP₂-interacting surface of the corresponding helix A-B linker superimposed with GPMI-P₂, and interactions between GPMI-P₂ and the top of the modeled corresponding mutant linker predicted by the docking simulation. Predicted favorable hydrogen bonds are indicated by dotted green lines. The interacting channel residues and the P1, P4, and P5 phosphates of GPMI-P₂ are labeled. All colors and symbols are as in Fig. 11.