Four basic residues shown to be critical for PIP₂ interactions in Kv7.2 are also critical for the corresponding residues of Kv7.3. Shown are single-channel records in the cell-attached (A) or inside-out patch (B) configurations of the indicated Kv7.3 wt or mutant channel at a membrane potential of 0 mV. The inside-out patch records are in the presence of 25 μM diC8-PIP₂. The traces labeled KKR-EEE are from a triple mutant consisting of K425E, K432E, and R434E. (C) Bars show summarized P_o values at 0 mV for both configurations for the indicated channel. Asterisks above each bar indicate the level of significance compared with the values for the corresponding wt channel. **, P < 0.01; ***, P < 0.001.