Figure 2. The exchange of carboxy termini between Kv7.3 and Kv7.4 wholly inverts their differential Po and PIP2-apparent affinities. The first chimera pair between Kv7.3 and Kv7.4 was created by exchange of the entire carboxy termini. Shown are single-channel patch-clamp recordings from the Kv7.3/7.4C-1 (A) or Kv7.4/7.3C-1 (B) chimeras obtained in the cell-attached (top) or excised inside-out (bottom) configurations at 0 mV. The indicated concentrations of diC8-PIP2 were perfused over the inside-out patches. The insets above the current records show the topography and chimera junctions of the channel subunits. (C) Bars summarize the Po at 0 mV in cell-attached patches for these chimeras. (D) Plotted are the summarized Po values at 0 mV for the chimeras in inside-out configuration obtained over a range of diC8-PIP2 concentrations. The data were fit by a Hill equation of the form and parameters given in the text.
Figure 2.

The exchange of carboxy termini between Kv7.3 and Kv7.4 wholly inverts their differential Po and PIP2-apparent affinities. The first chimera pair between Kv7.3 and Kv7.4 was created by exchange of the entire carboxy termini. Shown are single-channel patch-clamp recordings from the Kv7.3/7.4C-1 (A) or Kv7.4/7.3C-1 (B) chimeras obtained in the cell-attached (top) or excised inside-out (bottom) configurations at 0 mV. The indicated concentrations of diC8-PIP2 were perfused over the inside-out patches. The insets above the current records show the topography and chimera junctions of the channel subunits. (C) Bars summarize the Po at 0 mV in cell-attached patches for these chimeras. (D) Plotted are the summarized Po values at 0 mV for the chimeras in inside-out configuration obtained over a range of diC8-PIP2 concentrations. The data were fit by a Hill equation of the form and parameters given in the text.

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