Figure 1. Kv7.3 and 7.4 display highly differential Po, 0 mV values and PIP2-apparent affinities. Shown are single-channel patch-clamp recordings from a unitary Kv7.3 (A) or Kv7.4 (B) channel obtained in the cell-attached (top) or excised inside-out (bottom) configurations at 0 mV. The indicated concentrations of diC8-PIP2 were perfused over the inside-out patches. The insets above the current records show the topography of a channel subunit, with the A and B helices indicated. Here and throughout, Kv7.3 and Kv7.4 are indicated as red or blue, respectively. (C) Bars summarize the Po at 0 mV in cell-attached patches for Kv7.3 and Kv7.4. (D) Plotted are the summarized Po values at 0 mV for the channels in inside-out configuration, obtained over a range of diC8-PIP2 concentrations. The data were fit by a Hill equation of the form and parameters given in the text.
Figure 1.

Kv7.3 and 7.4 display highly differential Po, 0 mV values and PIP2-apparent affinities. Shown are single-channel patch-clamp recordings from a unitary Kv7.3 (A) or Kv7.4 (B) channel obtained in the cell-attached (top) or excised inside-out (bottom) configurations at 0 mV. The indicated concentrations of diC8-PIP2 were perfused over the inside-out patches. The insets above the current records show the topography of a channel subunit, with the A and B helices indicated. Here and throughout, Kv7.3 and Kv7.4 are indicated as red or blue, respectively. (C) Bars summarize the Po at 0 mV in cell-attached patches for Kv7.3 and Kv7.4. (D) Plotted are the summarized Po values at 0 mV for the channels in inside-out configuration, obtained over a range of diC8-PIP2 concentrations. The data were fit by a Hill equation of the form and parameters given in the text.

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