Figure 6.
![Figure 6. ATP and UTP-induced nitric oxide formation in astrocytes and its effect on neuronal SCO. (A) Increase in DAF fluorescence stimulated with 30 μM UTP in astrocytes in a mixed culture with and without pretreatment with 100 μM L-NAME. The traces are from single astrocytes. (B) Mean ± SEM. DAF fluorescence in 10–15 astrocytes from three experiments for each treatment. (C) SCO inhibition with UTP was prevented by pretreatment of cells with L-NAME or by 100 μM c-PTIO in the perfusate (D). (E) UTP induced [Ca2+]i rise in astrocytes in presence of c-PTIO. (F and G) Effect of nitric oxide donor DETA/NO on SCO frequency. Data in G are for 10–15 neurons for each data concentration from three experiments (**, P < 0.001).](https://cdn.rupress.org/rup/content_public/journal/jgp/132/3/10.1085_jgp.200810043/5/jgp_200810043_gs_fig6.jpeg?Expires=1767947614&Signature=Z3C5gJ8V0MJaTDrF02HtFEfnBK8xQ0LZ-lTV4B5bg7Yna4iz2yBMAp3OLZQOXUPU3aR6x3gv0-qYWk5I6J3U2uECFS6OeBg8xsi41bZ-zfVywyOCOyWnoSpNoxZ~asmEihH7SFWAJ1AMe8pa8J8ZXLnwYl8z29FszbfB85hK11YS7m1qQUaHCJ1ALt~lIr43Xa4hqLYChGnZN-boKNtLDdU7Xlj6cVzWWpAsgpsSTQ~N9cPYO-tcF1QvtpEZdacKnIX25iadH5UrdGClOf-PR8XEttPOEsOdT0Uz4QZer~ToazGTm4PmcmguT-XdJEMWEoRZnjj3Zcb9maKx6uJrog__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ATP and UTP-induced nitric oxide formation in astrocytes and its effect on neuronal SCO. (A) Increase in DAF fluorescence stimulated with 30 μM UTP in astrocytes in a mixed culture with and without pretreatment with 100 μM L-NAME. The traces are from single astrocytes. (B) Mean ± SEM. DAF fluorescence in 10–15 astrocytes from three experiments for each treatment. (C) SCO inhibition with UTP was prevented by pretreatment of cells with L-NAME or by 100 μM c-PTIO in the perfusate (D). (E) UTP induced [Ca2+]i rise in astrocytes in presence of c-PTIO. (F and G) Effect of nitric oxide donor DETA/NO on SCO frequency. Data in G are for 10–15 neurons for each data concentration from three experiments (**, P < 0.001).
