Figure 8.
Figure 8. Depolarization-induced calcium entry is sufficient to induce functional sAC-generated cAMP. (A) Cyclic AMP was measured in INS-1E cells after incubation for 15 min in 2.5 mM glucose (white bars) or 2.5 mM glucose plus 60 mM KCl (gray bars) in the presence of 0.5 mM IBMX, with either vehicle control, 50 μM 2′5′ ddAdo, and/or 30 μM KH7. Values represent means ± SEM (n ≥ 4). ANOVA statistical analyses were performed with the Bonferroni comparison test. ***, P < 0.001. (B) Western blots using anti-phosphoERK (pERK) or total ERK (ERK) of INS-1E cells incubated in Krebs-Ringer buffer with 2.5 mM glucose (LG) or 2.5 mM glucose with 60 mM KCl (LG+KCl), in the absence of IBMX, for 15 min in the presence or absence of 50 μM 2′5′ ddAdo or 30 μM KH7. Shown are representative experiments repeated four times.

Depolarization-induced calcium entry is sufficient to induce functional sAC-generated cAMP. (A) Cyclic AMP was measured in INS-1E cells after incubation for 15 min in 2.5 mM glucose (white bars) or 2.5 mM glucose plus 60 mM KCl (gray bars) in the presence of 0.5 mM IBMX, with either vehicle control, 50 μM 2′5′ ddAdo, and/or 30 μM KH7. Values represent means ± SEM (n ≥ 4). ANOVA statistical analyses were performed with the Bonferroni comparison test. ***, P < 0.001. (B) Western blots using anti-phosphoERK (pERK) or total ERK (ERK) of INS-1E cells incubated in Krebs-Ringer buffer with 2.5 mM glucose (LG) or 2.5 mM glucose with 60 mM KCl (LG+KCl), in the absence of IBMX, for 15 min in the presence or absence of 50 μM 2′5′ ddAdo or 30 μM KH7. Shown are representative experiments repeated four times.

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