Figure 6.
Figure 6. Glucose leads to ERK (p42/44) activation in a sAC-dependent manner. Western blots using anti-phosphoERK (pERK) or total ERK (ERK) of INS-1 cells incubated in Krebs Ringer buffer with 2.5 mM glucose (LG) or 16 mM glucose (HG) (in the absence of IBMX) (A) for 5, 10, 15, 20, and 30 min or (B) for 15 min in the presence or absence of 30 μM KH7 or 50 μM 2′5′ ddAdo or KH7 and 0.5 mM Sp-8Br-cAMP. Western blot shows phosphorylation and total protein for both ERK1 and ERK2, p44 and p42. Shown are representative experiments repeated multiple times (for A, n = 4; for B, n = 7).

Glucose leads to ERK (p42/44) activation in a sAC-dependent manner. Western blots using anti-phosphoERK (pERK) or total ERK (ERK) of INS-1 cells incubated in Krebs Ringer buffer with 2.5 mM glucose (LG) or 16 mM glucose (HG) (in the absence of IBMX) (A) for 5, 10, 15, 20, and 30 min or (B) for 15 min in the presence or absence of 30 μM KH7 or 50 μM 2′5′ ddAdo or KH7 and 0.5 mM Sp-8Br-cAMP. Western blot shows phosphorylation and total protein for both ERK1 and ERK2, p44 and p42. Shown are representative experiments repeated multiple times (for A, n = 4; for B, n = 7).

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