sAC is present in pancreatic β cells. (A) Western blot (WB) using biotinylated anti-sAC mAb R21 of an immunoprecipitation using anti-sAC mAb R37 or nonspecific mouse IgG from INS-1E cells. Specificity of the Western blot was confirmed using streptavidin alone (not depicted). (B) Immunocytochemistry of INS-1E cells using anti-sAC mAb R21 (green) alone (left top) or in combination with the nuclear stain, DAPI (blue) (right top). All specific staining was lost when the sAC mAb was preincubated with excess peptide antigen (bottom; left panel is fluorescence; right panel is bright field). Immunostains with secondary antibodies alone were also negative (not depicted).