Effects of Ni2+ on outward currents during large depolarizations, from the I-V protocol. Records are the ratio of currents recorded in Ni2+ to the average control currents, in Ba2+ (A) or Ca2+ (B and C), for 1 mM Ni2+ (A and B) or 3 mM Ni2+ (C). Ratios are blanked for 0.3–0.6 ms during the gating current. Voltage steps are in 20-mV increments. 3-kHz Gaussian filter, with 60-Hz noise subtracted for C only. A and B are from the same cells as Figs. 1 and 4, and C is from cell c050929.